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streptactin xt magnetic beads (IBA Lifesciences)

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IBA Lifesciences streptactin xt magnetic beads
Streptactin Xt Magnetic Beads, supplied by IBA Lifesciences, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xt+beads/MagStrep+Strep-Tactin+XT+beads/bio_rxiv__64898__2026__05__05__723063-203-21-25
Average 95 stars, based on 77 article reviews

streptactin xt magnetic beads - by Bioz Stars, 2026-10

95/100 stars

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Purification:

Article Title: A functional role for septin-2 in the maintenance of the axon initial segment and in human cognitive development.
Article Snippet: .. 30 Purification was carried out on MagStrep “Type3” XT beads (IBA Lifesciences) for 30 minutes 31 at 4oC, and washed 1X PBS, 1% Triton X100, followed by 2X PBS washes. ..

Article Title: Inositol hexakisphosphate Functions as a Cofactor and Modulator of ADAR1 Activity
Article Snippet: .. ADAR proteins were purified from the reaction mixture using MagStrep “type3” XT beads (Cat. # 2-4090-002, IBA) following the manufacturer’s instructions. ..

Article Title: Automated, modular assembly of reconstituted cell-free systems from in vitro -produced components
Article Snippet: Fluorescence of sfGFP was measured using a Hidex Sense 425-301 microplate reader, with excitation at 485 nm and emission at 535 nm. .. CuAAC reaction was carried out as described previously [ ]. ncAA-containing peptides were purified using MagStrep “type3” XT beads (IBA) and denatured using 0.1 % SDS in water. ..

Incubation:

Article Title: Synthetically designed anti-defense proteins overcome barriers to bacterial transformation and phage infection
Article Snippet: .. Tubes were centrifuged for 15 min at 15,000 g . Per each pellet, 20 μl of MagStrep ‘Type 3’ XT beads (IBA catalogue no. 2-4090-002) were washed in 200 μl wash buffer (IBA catalogue no. 2-1003-100), and the lysed cell supernatant was mixed with the beads and incubated for 60 min, rotating at 4 °C. ..

Suspension:

Article Title: Optimization of Replication, Transcription, and Translation in a Semi-Synthetic Organism
Article Snippet: Cell pellets collected from translation experiments were resuspended in BugBuster (100 μL 1×, EMD Millipore) and shaken at room temperature for 15 min. .. The cell lysate was then diluted with Buffer W (380 μL; 50 mM HEPES pH 8, 150mM NaCl, 1 mM EDTA) and magnetic Strep-Tactin beads (20 μL, 5% (v/v) suspension of MagStrep ‘type3’ XT beads, IBA Lifesciences) equilibrated in Buffer W, were added. ..

Article Title: Principles of cotranslational mitochondrial protein import
Article Snippet: .. 1.6 mL MagStrep “type3” XT beads (5% suspension, IBA Lifesciences, pre-washed for 3 times in Wash Buffer) was added to the remainder of the sample, and the suspension was rotated for 45 min at 4 °C. ..

Binding Assay:

Article Title: APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons
Article Snippet: .. Briefly, 50 μl of 0.2 μM Twin Strep tagged APC/C (containing wild-type or 3RE APC3 and harboring glutamate substitutions described above) in binding buffer 200 (20 mM HEPES pH 7.5, 200 mM NaCl, 10% glycerol, 1 mM DTT, 0.1% NP-40) were bound to 150 μl MagStrep “type3” XT beads (IBA Lifesciences) equilibrated in the same buffer on a platform shaker for 60 min at 4 °C. ..

Article Title: APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons.
Article Snippet: .. Briefly, 50 μl of 0.2 μM Twin Strep tagged APC/C (containing wild-type or 3RE APC3 and harboring glutamate substitutions described above) in binding buffer 200 (20mMHEPES pH 7.5, 200mMNaCl, 10% glycerol, 1mM DTT, 0.1% NP-40) were bound to 150 μl MagStrep “type3” XT beads (IBA Lifesciences) equilibrated in the same buffer on a platform shaker for 60min at 4 °C. ..

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Image Search Results

$Western blot (A) and silver-stained gel (B) show the purification fractions of the WT control (WT/WT), bait/WT experiment (bait/WT), and bait control (bait/bait), all crosslinked with 0.2% (w/v) FA. The fractions loaded are lysate (L) (1 μL), flowthrough (FT) (1 μL), wash steps 1 (W1) and 3 (W3) (8 μL), eluate (E) (4 μL), and residue (R) (4 μL). The Chameleon ® Due Pre-Stained protein marker was used for molecular weight reference. The position of the signals for PycA (127.72 kDa) and SigA-TS (45.9 kDa) is marked on the right side. PycA is a biotinylated protein and thus is purified unspecifically by the MagStrep beads.$

Journal: Bio-protocol

Article Title: TIE-UP-SIN: A Method for Enhanced Identification of Protein–Protein Interactions

doi: 10.21769/BioProtoc.5663

Figure Lengend Snippet: Western blot (A) and silver-stained gel (B) show the purification fractions of the WT control (WT/WT), bait/WT experiment (bait/WT), and bait control (bait/bait), all crosslinked with 0.2% (w/v) FA. The fractions loaded are lysate (L) (1 μL), flowthrough (FT) (1 μL), wash steps 1 (W1) and 3 (W3) (8 μL), eluate (E) (4 μL), and residue (R) (4 μL). The Chameleon ® Due Pre-Stained protein marker was used for molecular weight reference. The position of the signals for PycA (127.72 kDa) and SigA-TS (45.9 kDa) is marked on the right side. PycA is a biotinylated protein and thus is purified unspecifically by the MagStrep beads.

Article Snippet: MagStrep ® Strep-Tactin ® XT beads (IBA Lifesciences, catalog number: 2-5090-010) 14.

Techniques: Western Blot, Staining, Purification, Control, Residue, Marker, Molecular Weight